The Agricultural Branch Railroad was a railroad in Massachusetts. It was incorporated in 1847 to provide a rail connection between the towns of Framingham and Northborough through Southborough.
Service began on December 1, 1855 with 13.2 miles of track. A short branch off the main line from Marlborough Junction to Marlborough had also opened in June 1855.
In July 1866 a 14-mile extension opened from Northborough to the Fitchburg and Worcester Railroad at Pratts Junction in Sterling, through the towns of Berlin and Clinton.
On May 20, 1867, the name of the railroad was changed to the Boston, Clinton and Fitchburg Railroad, which operated until 1876 when it merged with the New Bedford Railroad to become the Boston, Clinton, Fitchburg and New Bedford Railroad.[1] The line later became part of the Old Colony Railroad network.